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dc.contributor.authorDahal, Neeti
dc.contributor.authorAbdelhamed, Hossam
dc.contributor.authorLu, Jingjun
dc.contributor.authorKarsi, Attila
dc.contributor.authorLawrence, Mark L.
dc.date.accessioned2015-10-09T19:50:51Z
dc.date.available2015-10-09T19:50:51Z
dc.date.issued6/7/2013
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11668/2513
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0065973
dc.description.abstractEdwardsiella ictaluri is a Gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (ESC). The disease causes considerable economic losses in the commercial catfish industry in the United States. Although antibiotics are used as feed additive, vaccination is a better alternative for prevention of the disease. Here we report the development and characterization of novel live attenuated E. ictaluri mutants. To accomplish this, several tricarboxylic acid cycle (sdhC, mdh, and frdA) and one-carbon metabolism genes (gcvP and glyA) were deleted in wild type E. ictaluri strain 93-146 by allelic exchange. Following bioluminescence tagging of the E. ictaluri ?sdhC, ?mdh, ?frdA, ?gcvP, and ?glyA mutants, their dissemination, attenuation, and vaccine efficacy were determined in catfish fingerlings by in vivo imaging technology. Immunogenicity of each mutant was also determined in catfish fingerlings. Results indicated that all of the E. ictaluri mutants were attenuated significantly in catfish compared to the parent strain as evidenced by 2,265-fold average reduction in bioluminescence signal from all the mutants at 144 h post-infection. Catfish immunized with the E. ictaluri ?sdhC, ?mdh, ?frdA, and ?glyA mutants had 100% relative percent survival (RPS), while E. ictaluri ?gcvP vaccinated catfish had 31.23% RPS after re-challenge with the wild type E. ictaluri.
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE (Volume 8, Issue 6)
dc.subject.otherAnimals
dc.subject.otherBacterial
dc.subject.otherBacterial: genetics
dc.subject.otherCarbon
dc.subject.otherCarbon: metabolism
dc.subject.otherCitric Acid Cycle
dc.subject.otherCitric Acid Cycle: genetics
dc.subject.otherEdwardsiella ictaluri
dc.subject.otherEdwardsiella ictaluri: genetics
dc.subject.otherEdwardsiella ictaluri: metabolism
dc.subject.otherEdwardsiella ictaluri: pathogenicity
dc.subject.otherEnterobacteriaceae Infections
dc.subject.otherEnterobacteriaceae Infections: immunology
dc.subject.otherEnterobacteriaceae Infections: microbiology
dc.subject.otherEnterobacteriaceae Infections: prevention & contro
dc.subject.otherFish Diseases
dc.subject.otherFish Diseases: immunology
dc.subject.otherFish Diseases: microbiology
dc.subject.otherFish Diseases: prevention & control
dc.subject.otherGenes
dc.subject.otherGenotype
dc.subject.otherIctaluridae
dc.subject.otherIctaluridae: immunology
dc.subject.otherIctaluridae: microbiology
dc.subject.otherImmersion
dc.subject.otherInjections
dc.subject.otherIntraperitoneal
dc.subject.otherLuminescent Measurements
dc.subject.otherSequence Deletion
dc.subject.otherSequence Deletion: genetics
dc.subject.otherSurvival Analysis
dc.subject.otherVaccination
dc.subject.otherVirulence
dc.subject.otherVirulence: genetics
dc.titleTricarboxylic acid cycle and one-carbon metabolism pathways are important in Edwardsiella ictaluri virulence.
dc.typeArticle
dc.publisher.departmentDepartment of Basic Sciences
dc.publisher.collegeCollege of Veterinary Medicine
dc.identifier.doi10.1371/journal.pone.0065973


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