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dc.contributor.authorShowmaker, Kurt
dc.contributor.authorLawrence, Gary W.
dc.contributor.authorLu, Shien
dc.contributor.authorBalbalian, Clarissa
dc.contributor.authorKlink, Vincent P.
dc.date.accessioned2015-10-09T19:50:47Z
dc.date.available2015-10-09T19:50:47Z
dc.date.issued12/16/2011
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11668/2496
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0028954
dc.description.abstractA quantitative PCR procedure targeting the ?-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE (Volume 6, Issue 12)
dc.subject.otherAnimals
dc.subject.otherBase Sequence
dc.subject.otherDNA
dc.subject.otherDNA Primers
dc.subject.otherDNA Primers: metabolism
dc.subject.otherGeographic Information Systems
dc.subject.otherHelminth
dc.subject.otherHelminth: analysis
dc.subject.otherHelminth: genetics
dc.subject.otherHelminth: isolation & purification
dc.subject.otherMetagenomics
dc.subject.otherMetagenomics: methods
dc.subject.otherMolecular Sequence Data
dc.subject.otherPolymerase Chain Reaction
dc.subject.otherReference Standards
dc.subject.otherSoil
dc.subject.otherSoil: parasitology
dc.subject.otherTylenchoidea
dc.subject.otherTylenchoidea: genetics
dc.titleQuantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
dc.typeArticle
dc.publisher.departmentDepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.collegeCollege of Agriculture and Life Sciences
dc.publisher.collegeCollege of Arts and Sciences
dc.identifier.doi10.1371/journal.pone.0028954
dc.publisher.researchcenterBost Extension Center


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