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dc.contributor.authorPaul, Debarati
dc.contributor.authorKumar, Ranjit
dc.contributor.authorNanduri, Bindu
dc.contributor.authorFrench, Todd
dc.contributor.authorPendarvis, Ken
dc.contributor.authorBrown, Ashli
dc.contributor.authorLawrence, Mark L.
dc.contributor.authorBurgess, Shane C.
dc.date.accessioned2015-10-09T19:50:46Z
dc.date.available2015-10-09T19:50:46Z
dc.date.issued2/28/2011
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11668/2492
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0017111
dc.description.abstractOligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium able to utilize CO and H(2) to derive energy for fixation of CO(2). Thus, it is capable of growth using syngas, which is a mixture of varying amounts of CO and H(2) generated by organic waste gasification. O. carboxidovorans is capable also of heterotrophic growth in standard bacteriologic media. Here we characterize how the O. carboxidovorans proteome adapts to different lifestyles of chemolithoautotrophy and heterotrophy. Fatty acid methyl ester (FAME) analysis of O. carboxidovorans grown with acetate or with syngas showed that the bacterium changes membrane fatty acid composition. Quantitative shotgun proteomic analysis of O. carboxidovorans grown in the presence of acetate and syngas showed production of proteins encoded on the megaplasmid for assimilating CO and H(2) as well as proteins encoded on the chromosome that might have contributed to fatty acid and acetate metabolism. We found that adaptation to chemolithoautotrophic growth involved adaptations in cell envelope, oxidative homeostasis, and metabolic pathways such as glyoxylate shunt and amino acid/cofactor biosynthetic enzymes.
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE (Volume 6, Issue 2)
dc.subject.otherBacterial
dc.subject.otherBacterial Proteins
dc.subject.otherBacterial Proteins: analysis
dc.subject.otherBradyrhizobiaceae
dc.subject.otherBradyrhizobiaceae: enzymology
dc.subject.otherBradyrhizobiaceae: genetics
dc.subject.otherBradyrhizobiaceae: growth & development
dc.subject.otherBradyrhizobiaceae: metabolism
dc.subject.otherChemoautotrophic Growth
dc.subject.otherChemoautotrophic Growth: physiology
dc.subject.otherCluster Analysis
dc.subject.otherFatty Acids
dc.subject.otherFatty Acids: analysis
dc.subject.otherGene Regulatory Networks
dc.subject.otherGene Regulatory Networks: physiology
dc.subject.otherGenes
dc.subject.otherGlyoxylates
dc.subject.otherGlyoxylates: metabolism
dc.subject.otherHeterotrophic Processes
dc.subject.otherHeterotrophic Processes: physiology
dc.subject.otherMembrane Lipids
dc.subject.otherMembrane Lipids: analysis
dc.subject.otherMetabolic Networks and Pathways
dc.subject.otherMetabolic Networks and Pathways: genetics
dc.subject.otherOxidation-Reduction
dc.subject.otherProteome
dc.subject.otherProteome: analysis
dc.titleProteome and membrane fatty acid analyses on Oligotropha carboxidovorans OM5 grown under chemolithoautotrophic and heterotrophic conditions.
dc.typeArticle
dc.publisher.departmentDepartment of Chemical Engineering
dc.publisher.departmentDepartment of Biochemistry and Molecular Biology
dc.publisher.collegeCollege of Veterinary Medicine
dc.publisher.collegeJames Worth Bagley College of Engineering
dc.publisher.collegeCollege of Agriculture and Life Sciences
dc.identifier.doi10.1371/journal.pone.0017111
dc.publisher.researchcenterLife Sciences and Biotechnology Institute


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