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dc.contributor.advisorMa, Din-Pow
dc.contributor.authorSoma, Shiva Theja Reddy
dc.date2013
dc.date.accessioned2020-09-16T21:11:44Z
dc.date.available2020-09-16T21:11:44Z
dc.identifier.urihttps://hdl.handle.net/11668/20311
dc.description.abstractThe ubiquitin-proteasome proteolysis pathway is responsible for the degradation of abnormal and short-lived proteins to regulate many important biochemical activities in eukaryotes. By employing affymetrix microarray analysis, we have identified a novel ubiquitin ligase E3 gene GhRING2 that is expressed differentially between two G. hirsutum lines - Texas Marker-1 and Chromosome Substitution Line CS-B25. The complete GhRING2 gene sequence was obtained by genomic and cDNA walking. The expression of GhRING2 in cotton fiber is developmentally regulated, suggesting that the ubiquitin-proteasome pathway may regulate cotton fiber growth and development. Using a yeast two-hybrid assay GhRING2 was found to interact with a PROTODERMAL FACTOR1 (GhPDF1) protein. GhPDF1 is expressed preferentially in immature ovules and fiber initials and the gene has been suggested to play a role in cell fate determination and fiber development. Pull down and plasmid swap assays were employed to confirm this interaction.
dc.publisherMississippi State University
dc.subject.otherProteasome
dc.subject.othermicroarray
dc.subject.otherubiquitin
dc.subject.otherfiber
dc.subject.otherligase
dc.subject.otherchromosome
dc.subject.othertwo-hybrid
dc.titleCharacterization of a Novel RING-type Ubiquitin E3 Ligase GhRING2 involved in Cotton Fiber Development
dc.typeThesis
dc.publisher.departmentDepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology
dc.publisher.collegeCollege of Agriculture and Life Sciences
dc.date.authorbirth1988
dc.subject.degreeMaster of Science
dc.subject.majorBiochemistry
dc.contributor.committeeJenkins, Johnie N.
dc.contributor.committeeSaha, Sukumar
dc.contributor.committeePeng, Zhaohua
dc.contributor.committeeLi, Jiaxu


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