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    Verticillium Dahliae Causes the Fungal Wilting Disease of Cotton Plants Grown on the Mississippi State North Farm

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    etd-10132015-123512.pdf (1.506 Mb )
    Author
    Albukhari, Fahad Mohammedsaleh
    Item Type
    Thesis
    Advisor
    Ma, Din-Pow
    Lu, Shien
    Committee
    Li, Jiaxu
    Metrics
    
    Abstract
    The emergence and spread of Verticillium wilt were observed in cotton plants at the R.R. Foil Plant Science Research Center at Mississippi State during the late summer in 2013 and 2014. Several fungi with different morphology and growth characteristics were isolated from diseased cotton plants. Genomic DNA was extracted from the isolated fungal species and used in molecular typing via PCR amplification and DNA sequencing analysis of the ribosomal internal transcribed spacer (ITS) region. A total of five fungal genera were identified, and Verticillium sp. was the most frequently isolated genus. The isolated Verticillium strains could be Verticillium dahliae, Verticillium longisporum or even Verticillium albo-atrum. A PCR-based genotyping method using VTA2 (Verticillium transcription activator) gene specific primers confirmed that the isolated Verticillium strain was Verticillium dahliae, and it caused Verticillium wilt in Mississippi cotton plants. Pathogenicity tests (Koch’s postulates) confirmed the earlier qualitative identifications of Verticillium dahliae in the greenhouse.
    Degree
    Master of Science
    Major
    Biochemistry
    College
    College of Agriculture and Life Sciences
    Department
    Department of Biochemistry, Molecular Biology, and Entomology and Plant Pathology
    URI
    https://hdl.handle.net/11668/19483
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    • Theses and Dissertations
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    Mississippi State University Libraries
    395 Hardy Rd
    P.O. Box 5408, Mississippi State, MS 39762-5408
    (662) 325-7668
    (662) 325-0011
    (662) 325-8183
    Contact repository admin Report a problem Terms of use Privacy policy Accessibility MSU Legal
     

     

    Mississippi State University Libraries
    395 Hardy Rd
    P.O. Box 5408, Mississippi State, MS 39762-5408
    (662) 325-7668
    (662) 325-0011
    (662) 325-8183
    Contact repository admin Report a problem Terms of use Privacy policy Accessibility MSU Legal