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dc.contributor.advisorMemili, Erdogan
dc.contributor.authorRobertson, LaShonda S. (LaShonda Shakita)
dc.date2009
dc.date.accessioned2020-08-28T16:40:42Z
dc.date.available2020-08-28T16:40:42Z
dc.identifier.urihttps://hdl.handle.net/11668/19403
dc.description.abstractMicroRNAs are small RNA molecules that could possibly play a major role in fertility. In the experiment, spermatozoa were extracted from bovine followed by an extraction of total RNA. Bovine spermatozoa were extracted from two bulls of different fertility, high and low fertility. An expression array was done to compare the expression levels of the microRNAs. It was shown that thousands of microRNAs are present in bovine spermatozoa but only a small amount was significantly expressed. The microRNAs from low fertility bulls were more highly expressed than those in high fertility bulls. A Bioanalyzer gel was used to confirm the results of the microarray data. The microRNAs were present in the bull’s spermatozoa at 25 nucleotides. The functions of the significantly expressed microRNAs are not known but there is a great possibility that their functions affect fertility.
dc.publisherMississippi State University
dc.subject.lccCattle--Fertility.
dc.subject.lccCattle--Reproduction.
dc.subject.lccCattle--Spermatozoa.
dc.subject.lccRNA.
dc.subject.othersmall RNAs
dc.subject.othermicroRNAs
dc.subject.otherfertility
dc.subject.otherreproduction
dc.subject.otherpiRNAs
dc.subject.otherbovine
dc.subject.othermicroarray
dc.titleIdentification of MicroRNAs in Bovine Spermatozoa with Implications of Fertility
dc.typeThesis
dc.publisher.departmentDepartment of Agriculture and Life Sciences.
dc.publisher.collegeCollege of Agricultural and Life Sciences
dc.date.authorbirth1984
dc.subject.degreeMaster of Science
dc.subject.majorAgriculture and Life Sciences with a concentration in Genetics
dc.contributor.committeePeebles, Edgar
dc.contributor.committeePeterson, Daniel
dc.contributor.committeeBolden-Tiller, Olga
dc.contributor.committeeBridges, Susan


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