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dc.contributor.advisorShan, Xueyan
dc.contributor.advisorMa, Din-Pow
dc.contributor.authorTiwari, Alka
dc.date2014
dc.date.accessioned2020-08-20T15:10:48Z
dc.date.available2020-08-20T15:10:48Z
dc.identifier.urihttps://hdl.handle.net/11668/18980
dc.description.abstractPlant breeders have focused on improving maize resistance to Aspergillus flavus infection and aflatoxin accumulation by breeding with genotypes having the desirable traits. Various maize inbred lines have been developed for the breeding of resistance. Identification of differentially expressed proteins among such maize inbred lines will facilitate the development of gene markers and expedite the breeding process. Computational biology and proteomics approaches on the investigation of differentially expressed proteins were explored in this research. The major research objectives included 1) application of computational methods in homology and comparative modeling to study 3D protein structures and identify single nucleotide polymorphisms (SNPs) involved in changes of protein structures and functions, which can in turn increase the efficiency of the development of DNA markers; 2) investigation of methods on total protein profiling including purification, separation, visualization, and computational analysis at the proteome level. Special research goals were set on the development of open source computational methods using Matlab image processing tools to quantify and compare protein expression levels visualized by 2D protein electrophoresis gel techniques.
dc.publisherMississippi State University
dc.subject.otherPyMOL
dc.subject.other3D structure
dc.subject.otherModeller
dc.subject.other2D gel electrophoresis
dc.subject.otherMatlab
dc.subject.otherProteomics
dc.subject.otherAflatoxin
dc.titleComputational Methods on Study of Differentially Expressed Proteins in Maize Proteomes Associated with Resistance to Aflatoxin Accumulation
dc.typeDissertation
dc.publisher.departmentDepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology
dc.publisher.collegeCollege of Agriculture and Life Sciences
dc.date.authorbirth1984
dc.subject.degreeDoctor of Philosophy
dc.subject.majorMolecular Biology
dc.contributor.committeeWilliams, W. Paul
dc.contributor.committeeBrown, Ashli
dc.contributor.committeePerkins, Andy D.


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