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dc.contributor.advisorFrench, W. Todd
dc.contributor.authorFord, Andrew Thompson
dc.date2012
dc.date.accessioned2020-06-30T17:17:24Z
dc.date.available2020-06-30T17:17:24Z
dc.identifier.urihttps://hdl.handle.net/11668/17977
dc.description.abstractWastewater has been shown to contain the necessary nutritive requirements for the growth of microorganisms. The term, oleaginous, has been given to a classification of microorganisms know to produce up to twenty percent of the weight as oil. This study is designed to examine the potential accumulation of lipids within an oleaginous consortium grown on synthetic wastewater. Potential of the fluorescent stain, Nile red, as a lipid detector is also emphasized. Percentages of extractables greater than thirty-five percent were achieved within the oleaginous consortium using a nitrogen-limited medium. Low pH was found to increase the percentages of extractables. Xylose was shown to be a more optimal carbon source for accumulation than glucose. Nile red was shown to bind to intracellular inclusions and may be useful in monitoring lipid accumulation in industrial settings.
dc.publisherMississippi State University
dc.subject.lcshLipids--Research.
dc.subject.lcshLipids--Biotechnology.
dc.subject.lcshMicroorganisms--Research.
dc.subject.otherBiodiesel
dc.subject.otherNile Red
dc.subject.otherYeast
dc.subject.otherOleaginous
dc.titleLipid Accumulation in Synthetic Wastewater-Grown Oleaginous Microorganisms
dc.typeThesis
dc.publisher.departmentDepartment of Biological Sciences
dc.publisher.collegeCollege of Arts & Sciences
dc.date.authorbirth1983
dc.subject.degreeMaster of Science
dc.contributor.committeeDonaldson, Janet
dc.contributor.committeeBrown, Lewis R.
dc.contributor.committeeHernandez, Rafael


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