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dc.contributor.advisorWang, Chinling
dc.contributor.authorMujahid, Sana.
dc.date2007
dc.date.accessioned2020-05-07T17:35:18Z
dc.date.available2020-05-07T17:35:18Z
dc.identifier.urihttps://hdl.handle.net/11668/17017
dc.description.abstractListeria monocytogenes is a deadly, Gram-positive foodborne pathogen that is ubiquitous in the environment. The bacterium expresses a number of virulence and stress adaptation proteins that support its pathogenic capabilities. Two-dimensional gel electrophoresis (2-DE) was used to map L. monocytogenes surface proteins, which play a central role in virulence, and to examine protein expression by L. monocytogenes grown on ready-to-eat meat, an important source of Listeria infections. A novel method for solubilization of surface proteins from L. monocytogenes for 2-DE was developed. Additionally, the unique proteome expressed by L. monocytogenes grown on a meat matrix was uncovered. The developed solubilization method will facilitate efforts to identify and routinely compare surface proteins of Listeria by 2-DE. Furthermore, the 2-DE database of proteins expressed by L. monocytogenes grown on a meat matrix will allow further understanding of the interactions of Listeria with its food environment that influence its ability to cause disease.
dc.publisherMississippi State University
dc.subject.lcshListeria monocytogenes--Metabolism.
dc.subject.lcshListeria monocytogenes--Molecular aspects.
dc.subject.lcshPathogenic microorganisms.
dc.subject.lcshBacterial proteins--Metabolism.
dc.subject.lcshProteomics.
dc.subject.lcshFoodborne diseases.
dc.subject.othertwo-dimensional gel electrophoresis
dc.subject.otherproteomics
dc.subject.otherListeria monocytogenes
dc.titleProteomic Analysis Of Listeria Monocytogenes
dc.typeThesis
dc.publisher.collegeCollege of Veterinary Medicine.
dc.subject.degreeMaster of Science
dc.subject.majorVeterinary Medical Science
dc.contributor.committeeBoyle, John
dc.contributor.committeeMa, Peter


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