A proteomic approach to profiling the pipping muscle of the broiler embryo
Sokale, Adebayo Oluwaseun
AdvisorPeebles, E. David
CommitteeKiess, S. Aaron
Willeford, O. Kenneth
The Musculus complexus (pipping muscle) plays a primary role in the hatching of the chick from the eggshell at the end of its embryonic life. Various metabolic and cellular changes have been associated with the pipping muscle during development. These studies profiled the pipping muscle at the molecular level by identifying proteins which are associated with the developmental changes. In the first phase of the study, protein expression profile of the Day 13 chicken embryo pipping muscle was obtained using DDF with nano HPLC mass spectrometric analyzer. Identified proteins were categorized based on Gene Ontology. In the second phase, pipping muscle lymph was profiled from Day 20 chicken embryo using PCT with LTQ-Orbitrap mass spectrometric analyzer. The identification of constituent proteins of the piping muscle provides a better understanding of the complex cellular processes and functionality of the pipping muscle with potential benefits for improving hatchability in the poultry industry.