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dc.contributor.advisorEmerson, Joseph P.en_US
dc.contributor.authorSaem, ASMen_US
dc.date.accessioned2019-07-18T21:29:27Zen_US
dc.date.available2019-07-18T21:29:27Zen_US
dc.date.issued8/9/2019
dc.identifier.urihttps://hdl.handle.net/11668/14572en_US
dc.description.abstractTransition metals are essential micronutrients for organisms. Excess zinc can be lethal to bacterial survival. Zinc homeostasis is crucial and is regulated by zinc uptake and efflux system. Streptococcus pneumoniae AdcR (Adhesin competence regulator) transcription factor is the member of MarR (multiple antibiotic resistance regulator) family proteins that suppress the zinc upregulation transporter. In the absence of zinc, AdcR has less affinity to bind DNA and uptake genes are expressed in order to transport zinc inside the cell. Streptococcus pneumoniae is a gram-positive human pathogen that is capable of colonizing in the human nasopharynx, especially in young children and leads to severe diseases including pneumonia, septicaemia, and meningitis. In this work, we have transformed AdcR gene in E.Coli (BL21D3) cell, and we have overexpressed and purified AdcR using classical molecular biology and protein purification techniques. We also performed preliminary biophysical characterization of purified zinc(II) dependent transcription factor AdcR.en_US
dc.language.isoen_USen_US
dc.publisherMississippi State Universityen_US
dc.titlePurification and preliminary characterization of Zinc(II) dependent transcription factor AdcRen_US
dc.typeGraduate Thesisen_US
dc.publisher.departmentDepartment of Chemistryen_US
dc.publisher.collegeCollege of Arts and Sciencesen_US
dc.source.institutionMississippi State Universityen_US
dc.subject.degreeMaster of Scienceen_US
dc.subject.majorChemistryen_US
dc.contributor.committeeFitzkee, Nicholas C.en_US
dc.contributor.committeeMlsna, Debra Annen_US
dc.rights.embargodescriptionVisible MSU Only 3 Years
dc.rights.embargoliftdate08/15/2022


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