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dc.contributor.advisorGordon, Donna M.en_US
dc.contributor.authorBoth, Andries Pieteren_US
dc.description.abstractPcp1p is a rhomboid protease in the inner mitochondrial membrane of S. cerevisiae that processes Mgm1p, a protein required for mitochondrial fusion. In this study, five previously generated pcp1 mutant strains were analyzed for mitochondrial respiration, genome maintenance, and tubularization. We found that mutations that introduced a charged residue into Pcp1p resulted in a higher degree of respiratory deficiency, mtDNA loss, mitochondrial fragmentation, and possible mitochondrial membrane potential loss. Furthermore, these mutant phenotypes paralleled an increase in an aberrant form of Mgm1p that accumulates in the absence of wild type Pcp1p. This raises questions about the biogenesis of aberrant Mgm1p and its involvement in disrupting normal mitochondrial fusion. The findings outlined herein will help to establish a cascade of events that lead from loss of proper Mgm1p cleavage to associated cellular defects. Complete understanding of these connections will be valuable in establishing a similar cascade in the human disease ADOA.en_US
dc.publisherMississippi State Universityen_US
dc.publisherJudy and Bobby Shackouls Honors Collegeen_US
dc.relation.ispartofseriesJudy and Bobby Shackouls Honors College Undergraduate Senior Thesesen_US
dc.subject.lcshUniversities and colleges--Honors coursesen_US
dc.subject.otheramino acid mutationsen_US
dc.titleImpact of single amino acid mutations in the rhomboid protease Pcp1p on Pcp1p catalytic activity, cellular respiration, mitochondrial morphology, and mitochondrial DNA maintenanceen_US
dc.typeHonors Thesisen_US
dc.publisher.departmentDepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathologyen_US
dc.publisher.collegeJudy and Bobby Shackouls Honors Collegeen_US
dc.publisher.collegeCollege of Agriculture and Life Sciencesen_US
dc.publisher.officeHonors Office of Undergraduate Researchen_US
dc.contributor.issuingbodyMississippi State Universityen_US
dc.source.institutionMississippi State Universityen_US
dc.subject.degreeBachelor of Scienceen_US
dc.contributor.committeeHoffman, Federicoen_US
dc.contributor.committeeOppenheimer, Seth F.en_US

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  • Honors Theses
    Theses written by students of the Judy and Bobby Shackouls Honors College.

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