The in vitro effects of glucosamine on equine and canine platelet aggregation and thromboxane synthesis
MetadataShow full item record
Citrated blood samples were collected via jugular venipuncture and used to create platelet-rich plasma (PRP). PRP samples were incubated for 5 minutes with four concentrations of glucosamine: 0 �g/ml (control), 10 �g/ml, and 100 �g/ml. Platelet aggregation was determined using turbidometric aggregometry using ADP or collagen as agonists. Thromboxane B2 concentrations were measured using a commercially available ELISA kit. The results were analyzed using mixed model analysis. Significance was set at p<0.05.